Merck, 10127965001, NAD

General description
Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD⁺ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.
Application
NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.
NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.
Biochem/physiol Actions
Electron acceptor
The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.
NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.
Features and Benefits
Absorbance coefficients: 17.6 x 10³l x mol⁻¹ x cm⁻¹ at 260nm, 5.9 x 10³l x mol⁻¹ x cm⁻¹ at 333nm (CN complex)
Packaging
Packaged by solid weight.
Preparation Note
Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C
Analysis Note
Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A₂₅₀nm - A₂₆₀nm : 0.83 ± 0.02, A₂₈₀nm - A₂₆₀nm : 0.22 ± 0.02
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)
Other Notes
For life science research only. Not for use in diagnostic procedures.
This is the common form of NAD.