Description
General description
Coliforms 100 is a chromogenic medium that provides easy-to-read, definitive positive results. It has a high nutritional quality of the peptones along with incorporated phosphate buffer that guarantees rapid growth of coliforms, while lauryl sulfate largely inhibits the accompanying flora (especially the Gram-positive). The chromogenic substrates X-GAL (cleaved by coliforms) and the fluorogenic substrate MUG (highly specific for E. coli) allow for the simultaneous detection of total coliforms and E. coli. The presence of total coliforms is indicated by a blue-green color of the broth and E. coli by a blue fluorescence under UV-light. The optional 30-second indole reaction helps to accurately confirm the presence of E. coli. ReadyCult® tests are convenient, rapid and economical.
Application
Coliforms 100 is a rapid E. coli/Coliform test for drinking water. It is a selective enrichment broth used for the simultaneous presence / absence detection of total coliforms and E. coli within the bacteriological water examination.
Analysis Note
Appearance
- clearness:
clear
- colour: yellowish
pH-value (25 °C): 6.6 - 7.0
Inoculum on reference medium
- Escherichia coli ATCC 11775 (WDCM 00090):
10 - 100
- Citrobacter freundii ATCC 8090: 10 - 100
- Klebsiella pneumoniae ATCC 31488: 10 - 100
- Salmonella typhimurium ATCC 14028 (WDCM 00031): 10 - 100
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): ≥ 1000
Growth
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: +
- Klebsiella pneumoniae ATCC 31488: +
- Salmonella typhimurium ATCC 14028 (WDCM 00031): +
colour change to blue-green
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: +
- Klebsiella pneumoniae ATCC 31488: +
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): -
Fluorescence
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: -
- Klebsiella pneumoniae ATCC 31488: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
- Pseudomonas aeruginosa ATCC 10145 (WDCM 00024): -
Indole formation
- Escherichia coli ATCC 11775 (WDCM 00090):
+
- Citrobacter freundii ATCC 8090: -
- Klebsiella pneumoniae ATCC 31488: -
- Salmonella typhimurium ATCC 14028 (WDCM 00031): -
Incubation: 24 hrs.; 35 °C; aerobic.
Other Notes
Replaces: 1012980002
Legal Information
READYCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
