Merck, 1021290001, Chromolith® RP-18 endcapped

Name: Chromolith® RP-18 endcapped
Brand: Merck
SKU: 1021290001

Size:1 unit, Chromolith® Performance RP-18 endcapped 100-4.6 HPLC column, L × I.D. 100 mm x 4.6 mm HPLC column

Catalog Number: 1021290001

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Category

Reversed-Phase HPLC Columns

Pack Size1 units

TagsHPLC Columns Analytical Chemistry Analytical Chromatography

CollectionsMerck Analytical Chromatography Products & Lab Supplies Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® Performance RP-18 endcapped HPLC columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å) and the surface modification is octadecylsilane with full end-capping.

Chromolith® Performance RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column.

Chromolith®Performance RP-18 endcapped columns are perfectly suitable for LC-MS applications. The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:

Rapid separations at very low column back-pressure
High matrix tolerance, allowing time and cost savings with simplified workflows
Cost savings due to extended column lifetimes
Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are: Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032] Or Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51451] with the Chromolith® Guard Cartridge Holder 10 mm x 4.6 mm [1.52033] The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467] Need more efficiency? Chromolith®HighResolution RP-18 endcapped 100-4.6 HPLC columns [1.52022] provide a column efficiency exceeding 140,000 plates/meter. Learn more: Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments. Supelco HPLC Products: The Best Choice for Any LC Instrument
Application

Salt pollution reduces turbidity, dissolved organic matter, and cyanobacteria in experimental vernal pool communities: Discusses environmental applications of calcium chloride, focusing on its use in managing ecosystems affected by salt pollution, demonstrating its role in environmental remediation and management. (Vigil and Schuler, 2024).

Analysis Note
Appearance: conforms
Carbon content: 17,0 - 18,6 %
Pressure: ≤ 30 bar
stability testing: conforms
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3,9 - 4,6
Tailing (USP): 0,90 - 1,60 %
Selectivity
separation factor (anthracene/progesterone): 1,95 - 2,35
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Specifications

General

UNSPSC Code	41115709
Web Name Suffix Override	L × I.D. 100 mm x 4.6 mm HPLC Column
Web Name Override	Chromolith® RP-18 endcapped (monolithic) HPLC Columns
NACRES	SB.52
material	PEEK column
agency	suitable for USP L1
product line	Chromolith®
feature	endcapped
packaging	1 ea of
extent of labeling	18% Carbon loading
parameter	200 bar pressure
technique(s)	HPLC: suitable
column L × I.D.	10 cm × 4.6 mm
matrix	monolithic silica
matrix active group	C18 bonding phase
pore size	13 nm mesoporosity
operating pH range	2-7.5
separation technique	reversed phase
storage temp.	no temp limit
Quality Level	100

FAQs

Question: What is distinctive about Chromolith RP-18 compared with conventional packed columns?

Answer:It uses a continuous monolithic silica bed with large flow-through macropores instead of packed particles.

Question: What are the column dimensions?

Answer:The analytical column is 100 mm long with a 4.6 mm internal diameter.

Question: What bonded chemistry is used?

Answer:The column uses an endcapped C18 reversed-phase stationary phase.

Application Note

Chromolith® RP-18 endcapped is used for reversed-phase HPLC separations that benefit from the low backpressure and high permeability of monolithic silica. This 100 × 4.6 mm analytical column contains a continuous monolithic silica rod rather than packed particles. The structure combines approximately 2 µm macropores for convective mobile-phase flow with about 13 nm mesopores that provide chromatographic surface area. The stationary phase carries endcapped C18 chemistry for hydrophobic reversed-phase retention of small molecules. Because the monolithic bed generates lower pressure than conventional packed columns at comparable dimensions, higher flow rates can be used to shorten analysis time while maintaining useful separation efficiency. The format is suited to routine HPLC, method development and high-throughput work where rapid separations and reduced pressure demand are beneficial.

Ordering

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