Description
General description
Envirocheck™ Contact Yeasts and Molds offer a semi-quantitative method to detect microorganisms on surfaces and in liquids. The flexible paddles are coated with Tryptic Soy Agar for yeast and mold analysis. Slide A uses CASO (Tryptic Soy) Agar with 0.05 % Triphenyl Tetrazolium chloride (TTC), where red colonies indicate bacterial growth through the formation of red formazan dye upon TTC reduction. Slide B is coated with Rose Bengal Chloramphenicol Agar, which inhibits bacterial growth while reducing the size and height of mold colonies, preventing overgrowth of slower-growing fungi by faster-growing species.
Application
Envirocheck™ Contact YM(R) slides can be used for the detection of yeasts and molds on bothsurface and liquid testing from environmental materials and foodstuffs. The contact slides are suitable for industrial and environmental monitoring.
Features and Benefits
Avoid freezing. Frozen agar shows a dimpled or fuzzy surface and cannot be used anymore.
Condensation can be prevented by avoiding quick temperature shifts and mechanical stress.
Upon storage, the dip slides should not be placed near heat sources such as refrigerators with heat-emitting condensers.
Analysis Note
Count the number of colonies on both sides of the paddle. CASO Agar with TTC: Count the number of colonies for obtaining the total aerobic bacterial count (TVC). Rose Bengal Chloramphenicol Agar: Count the number of colonies to obtain the fungal contamination (colonies of yeasts are red-pink)
Sterility: passes test
pH-value/side one (25 °C): 6.6 - 7.3
pH-value/side two (25 °C): 6.2 - 7.0
Growth/side one
- Escherichia coli ATCC 8739 (WDCM 00012):
good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): fair/good
- Candida albicans ATCC 10231 (WDCM 00054): none/poor
- Saccharomyces cerevisiae ATCC 9763 (WDCM 00058): none/poor
- Pseudomonas aeruginosa ATCC 9027 (WDCM 00026): good
- Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053): good
Growth/side two
- Escherichia coli ATCC 8739 (WDCM 00012):
none
- Staphylococcus aureus ATCC 6538 (WDCM 00032): none
- Candida albicans ATCC 10231 (WDCM 00054): good
- Saccharomyces cerevisiae ATCC 9763 (WDCM 00058): good
- Pseudomonas aeruginosa ATCC 9027 (WDCM 00026): inhibited
- Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053): good
Incubation: 48 hrs.; 28 °C; aerobic; Aspergillus up to 72 hrs
Legal Information
ENVIROCHECK is a trademark of Merck KGaA, Darmstadt, Germany
