Merck, 10269638001, Collagenase/Dispase®</SUP>

General description
Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact. Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10 °C.
Application
Collagenase/Dispase® is used for the preparation of cells from a wide variety of different tissues and organs.
Biochem/physiol Actions
Collagenase degrades native collagen. Dispase® is a nonspecific protease.
Preparation Note
Activator: Ca²⁺

Working concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)

Enzyme activity:
Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Dispase®: 0.8 Wünsch U/mg lyophilizate (+37°C, casein as substrate, pH 7.5)
Contaminating proteolytic activities: Note: Store in appropriate aliquots and avoid repeated freezing and thawing!
Reconstitution in water (100mg/mL). Further dilution with PBS (phosphate buffered saline) (Ca²⁺/Mg²⁺-free).
Store dry
Other Notes
Collagenase from Vibrio alginolyticus, Dispase® from Bacillus polymyxa
EC 3.4.24.4 & 3.4.24.3
For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity, which liberates under assay conditions within 1 minute Folin-positive amino acids and peptides corresponding to 1 μmol tyrosine.
Legal Information
Dispase is a registered trademark of Godo Shusei Co., Ltd.