| NACRES | NA.74 |
|---|---|
| UNSPSC Code | 41106213 |
| agency | SMWW Part 9230 |
| sterility | non-sterile |
| form | granular |
| packaging | pkg of 500 g |
| manufacturer/tradename | GranuCult® prime |
| storage condition | dry media (Keep tightly closed) |
| technique(s) | microbiological culture: suitable |
| color | beige |
| pH | 7.2 (35 °C, 41 g/L in H2O) |
| solubility | 41.5 g/L |
| bulk density | 580 kg/m3 |
| application(s) | food and beveragesmicrobiology |
| storage temp. | 15-25°C |
| suitability | Enterococcus spp. |
| Quality Level | 200 |
Answer:It is used to enumerate enterococci in water and other liquids by membrane filtration.
Answer:Enterococci reduce TTC to red formazan.
Answer:The product is supplied as 500 g of non-sterile granular medium.
SLANETZ and BARTLEY Agar with TTC is designed for selective enumeration of enterococci in water and other liquids using membrane filtration. This GranuCult® prime dehydrated medium is supplied as 500 g of granules and aligns with ISO 7899 and Standard Methods Part 9230. Sodium azide in the formulation inhibits much of the accompanying Gram-negative flora, while enterococci reduce 2,3,5-triphenyltetrazolium chloride (TTC) to red formazan, producing characteristic red colonies. Tryptose supplies amino acids and nitrogen, yeast extract supplies vitamins, glucose acts as a carbon source and phosphate helps buffer the medium. The recommended preparation concentration is approximately 41.5 g/L, producing a pH around 7.2 under the stated conditions. After an aqueous sample is filtered, the membrane can be transferred onto the prepared agar for incubation and colony enumeration under the applicable method. The product is a non-sterile dry medium intended for preparation before use. Store the tightly closed granules dry at 15–25 °C. The TTC-containing selective formulation distinguishes this medium from a bile-esculin confirmation agar.
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