General description25 Glass plates 5 x 10 cm
HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.
Application
- Stability indicating high performance thin layer chromatography method development and validation for quantitative determination of tetracycline hydrochloride: In this study, the use of Silica gel F₂₅₄ HPTLC plates is optimized for analyzing tetracycline hydrochloride in pharmaceutical forms. This method serves as a stability-indicating test, crucial for ensuring the safety and efficacy of this common antibiotic, highlighting the material′s importance in regulatory compliance and drug development (Gashaw et al., 2024).
- Robust high-performance thin-layer chromatography (HPTLC) method for stability assessment and simultaneous quantification of epigallocatechin-3-gallate and rosmarinic acid in lipid-based nanoparticles and biological matrices: This research uses Silica gel F₂₅₄ HPTLC plates to develop a high-performance chromatographic method for assessing the stability and concentration of bioactive compounds in complex samples. The technique is essential for advanced material science and biomedical research, providing accurate and reproducible results (Koli et al., 2024).
Analysis NoteSpecific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steriod test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)
Other NotesReplaces: 105616-2; 105616
