Merck, 1057380001, TLC plates, Kieselguhr F₂₅₄

General description
25 Glassplates 20 x 20 cm
Precoated Kieselguhr or mixed Silica gel/Kieselguhr plates are only of small importance nowadays. Kieselguhr layers are used like cellulose layers for the TLC separation of polar and moderately polar substances. Polyamide, on the other hand , is used for the separation of flavonoids and other phenolic substances.
Analysis Note
Layer thickness: 180 - 220 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testing:
amino acid test
hRf-values
- β-alanine, short-time test:
20 - 30
- L-alanine, amino acid test: 40 - 50
- DL-Valin, amino acid test: 75 - 85
- L-leucine, amino acid test: 88 - 95
Typical value determined on a plate
Eluent: n- propanol/ water (80/20 v/v)
Other Notes
Replaces: 5738-7; 5738