Description
General description
The Pseudomonas CN Selective Supplement is a mixture of 2 different inhibitors in lyophilized form. Cetrimide and Nalidixic acid inhibit the accompanying Gram-positive and Gram-negative flora.
Application
Pseudomonas CN Selective Supplement is an additive for the preparation of Pseudomonas CN Selective Agar (Base) (1.07620) for the detection and enumeration of Pseudomonas aeruginosa in water by the membrane filter technique.
Analysis Note
Recovery on test medium
- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025):
≥ 70 %
- Aeromonas hydrophila ATCC 7966 (WDCM 00063): ≤ 0.01 %
- Klebsiella pneumoniae ATCC 13883 (WDCM 00097): ≤ 0.01 %
- Proteus mirabilis ATCC 14153: ≤ 0.01 %
- Providencia rustigianii ATCC 13159: ≤ 0.01 %
Colonies (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): greenish
Growth (Pseudomonas fluorescens ATCC 13525 (WDCM 00115)): inhibited
Incubation: 44 hrs.; 35 °C; aerobic.
Reference: blood-agar (24 hrs.; 30 °C; aerobic).
Supplement tested in Pseudomonas selektiv agar (base) art. no. 1.07620.
Other Notes
Replaces: 1.07624.0001