Merck, 10799050001, BM-Cyclin

General description
BM-Cyclin is a combination of two antibiotics, namely tiamulin and minocycline, both of which prevent protein synthesis.
Application
BM-Cyclin is used for the elimination of mycoplasma from infected cell cultures without obvious marked cytotoxic side effects.
Biochem/physiol Actions
Sensitive Organisms: Mycoplasmas and bacteria

BM Cyclin was found to effectively eliminate Acholesplasma laidlawii,
Mycoplasma arginini,
Mycoplasma hyorhinis, and
Mycoplasma orale
from experimentally contaminated and chronically infected cell lines.
Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures.
Packaging
Set of two different antibiotics
Physical form
Vial 1 (Tiamulin-hydrogenfumarat): clear, colorless solution after reconstitution
Vial 2 (Minocyclinhydrochloride): clear, yellow solution after reconstitution
Preparation Note
Working concentration: 10μg/ml pleuromutilin derivative (BM-cyclin 1), 5μg/ml tetracycline derivative (BM-cyclin 2).
Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines.
For hybridomas decrease concentration of BM-Cyclin by 50%.
Preparation of DAPI Working Solution
Dilute the stock solution with methanol to a final concentration of 1μg/ml.
Working solution: Preparation of Stock Solution
Dissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water.
Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock Solution
Dissolve DAPI in water to a final concentration of 1 to 5mg/ml.
Note: Do not use any buffers.
This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots.
Storage conditions (working solution): -15 to -25°C
Reconstituted stable at -15 to -25°C for at least 6 months.
Other Notes
For life science research only. Not for use in diagnostic procedures.