Merck, 10810118001, CHAPS

Name: CHAPS
Brand: Merck
SKU: 10810118001

Size:10 g, CHAPS, 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate, 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate, zwitterionic, Cholamidopropyl-dimethyl-ammonio]-1-propane sulfonate, 3-3-, zwitterionic, cholamidopropyl-dimethyl-ammonio]-1-propane sulfonate, 3-3-, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate, 98% (from N), suitable for dialysis, solubility: >50% (Aqueous solutions)

Catalog Number: 10810118001

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Category

Dialysis Membranes & Cassettes

Pack Size10 g

TagsChemistry & Biochemicals Biochemicals Detergents

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
3-[(3-Cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate
Application
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
Solubilization of membrane proteins. Combines the properties of both the sulfobetaine-type and bile-acid detergents.
Physical form
Crystals
Preparation Note
Working concentration: PAGE: 6.5 to 13 mM
Storage conditions (working solution): Do not store in solution, because precipitation may occur.
Other Notes
For life science research only. Not for use in diagnostic procedures.

Specifications

General

UNSPSC Code	12161900
MDL number	MFCD00012116
NACRES	NA.54
InChI key	UMCMPZBLKLEWAF-RFCNGIAKSA-N
InChI	1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
SMILES string	C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
description	zwitterionic
assay	98% (from N)
form	crystals
mol wt	micellar avg mol wt 6150
packaging	pkg of 10 g (10810118001)
manufacturer/tradename	Roche
aggregation number	10
technique(s)	dialysis: suitable
CMC	4 mM
transition temp	cloud point >100 °C
solubility	>50% (Aqueous solutions)
absorption	Note:* Arbitrary scale ranges from ++ to −)
storage temp.	2-8°C

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Merck, 10810118001, CHAPS

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