Description
General description
GranuCult® plus LB broth (MILLER) formulation supports the growth of Escherichia coli. Casein peptone and yeast extracts supply essential growth factors, such as nitrogen, carbon, sulfur, minerals, and vitamins.
Application
GranuCult® plus LB broth (MILLER) is suitable for the cultivation of E. coli in fermentation and molecular genetic studies.
Features and Benefits
GranuCult® offers superior granulated culture media
Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
Excellent wettability, solubility, and free-flowing properties
Convenient, with minimal component separation and clumping, even under warm or humid conditions
High batch-to-batch reproducibility
Prolonged shelf life of up to five years
High number of test strains exceeding all regulatory demands
Granulation technology allows many supplements to be included, with no need to add these separately
Analysis Note
Appearance (clearness): clear
Appearance (color): yellowish to yellowish brown
pH-value (25 °C): 6.8 - 7.2
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 100
Inoculum on reference medium (Escherichia coli DSM 6897 (DH5alpha)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 47092 (BL21)): ≤ 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good to very good
Growth (Escherichia coli DSM 6897 (DH5alpha)): good to very good
Growth (Escherichia coli ATCC 47092 (BL21)): good to very good
Incubation: 24 ± 2 hours at 37 ± 1 °C, aerobic
Other Notes
Replaces: 1.10285.5007
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
