Merck, 11033105-1VL, HeLa9903

Biochem/physiol Actions
HeLa, genetic modification, the hER alpha expression construct, Oestrogen-Responsive Element
HeLa9903 is a HeLa cell line stably transfected with two constructs: the hER alpha expression construct; a firefly luciferase reporter construct bearing Oestrogen-Responsive Element (ERE) driven by a mouse metallothionein (MT) promoter TATA element. HeLa9903 can be used to test for evaluation of chemicals based on OECD Test Guideline: The stably transformed human oestrogen receptor-alpha transcriptional; activation assay for detection of oestrogenic activity of chemicals.
STR-PCR Data:
Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Preparation Note
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 16μg/ml Blasticidin S HCL + 800μg/ml G418.
Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 2 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].
Legal Information
US7537904B1 N Ooe & Matsunaga H 2009.Cell for measuring the ability to control the activity of a ligand-responsive transcription control factor.