Description
General description
BHI (Brain Heart Infusion) broth is a granulated selective medium recommended for the cultivation of various fastidious bacteria and the cultivation of Staphylococci for the plasma coagulase test. The granules ensure rapid and uniform dissolution in water. It also prevents clumping of the medium and inhalation of the airborne powder. Enzymatic digest of animal tissues and infusions (calf brain and beef heart) serve as sources of carbon, nitrogen, essential growth factors, amino acids, and vitamins. Glucose serves as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium, whereas sodium chloride maintains the osmotic equilibrium of the medium.
Application
BHI (Brain Heart Infusion) broth is a highly nutritious and buffered medium to support the growth of fastidious and non-fastidious microorganisms, including aerobic and anaerobic bacteria.
Analysis Note
Appearance (colour): brown
Appearance (clearness): clear
pH-value (25 °C): 7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Inoculum on reference medium (Streptococcus pneumoniae ATCC 6305): ≤ 100
Inoculum on reference medium (Streptococcus pyogenes ATCC 19615): ≤ 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 100
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): ≤ 100
Inoculum on reference medium (Bacteroides fragilis ATCC 25285): ≤ 100
Inoculum on reference medium (Haemophilus influenzae ATCC 33391): ≤ 100
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): good to very good
Growth (Streptococcus pneumoniae ATCC 6305): good to very good
Growth (Streptococcus pyogenes ATCC 19615): good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): good to very good
Growth (Candida albicans ATCC 10231 (WDCM 00054)): good to very good
Growth (Bacteroides fragilis ATCC 25285): good to very good
Growth (Haemophilus influenzae ATCC 33391): good to very good
Incubation:
24 ± 2 hours at 37 ± 1 °C aerobic
C.albicans 44 ± 4 hours at 37 ± 1 °C aerobic
B.fragilis 44 ± 4 hours at 37 ± 1 °C anaerobic
H.influenza 44 ± 4 hours at 37 ± 1 °C microaerophilic and with addition of X+V-factor.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
