Description
General description
GranuCult® prime MRS agar (de MAN, ROGOSA and SHARPE) is a Lactobacillus selective medium. This medium contains polysorbate, acetate, magnesium, and manganese, which act as special growth factors for Lactobacilli, as well as a rich nutrient base. As the medium exhibits a low degree of selectivity, it may support the growth of Pediococcus and Leuconostoc species and other secondary bacteria.
Application
GranuCult® prime MRS agar is suitable for isolation, enumeration, and cultivation of Lactobacillus spp. and other mesophilic lactic acid bacteria from all types of materials.
Features and Benefits
GranuCult® offers superior granulated culture media.
Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
Excellent wettability, solubility, and free-flowing properties
Convenient, with minimal component separation and clumping, even under warm or humid conditions.
High batch-to-batch reproducibility
Prolonged shelf life of up to five years
High number of test strains exceeding all regulatory demands
Granulation technology allows many supplements to be included, with no need to add these separately
Analysis Note
Appearance (clearness): clear
Appearance (colour): brown
pH-value (25 °C): 5.6 - 5.8
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)):
Inoculum on reference medium (Lactobacillus sakei ATCC 15521 (WDCM 00015)):
Inoculum on reference medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)):
Inoculum on reference medium (Pediococcus pentosaceus ATCC 33316 (WDCM 00158)):
Inoculum on reference medium (Pediococcus damnosus ATCC 29358 (WDCM 00022)):
Colony count (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)):
Colony count (Lactobacillus sakei ATCC 15521 (WDCM 00015)):
Colony count (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)):
Colony count (Pediococcus pentosaceus ATCC 33316 (WDCM 00158)):
Colony count (Pediococcus damnosus ATCC 29358 (WDCM 00022)):
Recovery on test medium (Lactobacillus sakei ATCC 15521 (WDCM 00015)): ≥ 70 %
Recovery on test medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)): ≥ 70 %
Recovery on test medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)): ≥ 70 %
Recovery on test medium (Pediococcus pentosaceus ATCC 33316 (WDCM 00158)): ≥ 70 %
Recovery on test medium (Pediococcus damnosus ATCC 29358 (WDCM 00022)): ≥ 70 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): total inhibition
Growth (Bifidobacterium bifidum ATCC 11863): good growth
Incubation: 72 ± 3 hours at 30 ± 1 °C aerobic; Bifidobacterium anaerobic
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: MRS-Agar
Other Notes
Do not use clumped or discolored medium.
The prepared medium is clear and brown.
Prepared plates can be stored at +2 °C to + 8 °C °C in the dark and protected against evaporation for up to 14 days.
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
