Description
General description
Differential Reinforced Clostridial Broth is used to determine the count of sulfite-reducing bacteria by the MPN technique. The redox indicator resazurin is used to monitor anaerobiosis. Clostridia reduce sulfite to sulfide, which causes the culture medium to turn black. As other bacteria can also produce sulfide, vegetative forms must first be removed from the culture by a relevant treatment (e.g., pasteurization), and the anaerobic spore-forming microorganisms must then be identified. The addition of polymyxin (70 IU/ml) to the broth inhibits the growth of most non-spore-forming microorganisms.
Application
Differential reinforced clostridial broth is suitable for the enumeration of all clostridia by the MPN method in foodstuffs and other materials.
Features and Benefits
GranuCult® offers superior granulated culture media
Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
Excellent wettability, solubility, and free-flowing properties
Convenient, with minimal component separation and clumping, even under warm or humid conditions
High batch-to-batch reproducibility
Prolonged shelf life of up to five years
High number of test strains exceeding all regulatory demands
Granulation technology allows many supplements to be included, with no need to add these separately
Analysis Note
Appearance (clearness): clear
Appearance (colour): reddish-brown
pH-value (25 °C): 6.9 - 7.3
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): fair to good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): poor to fair
Growth (Clostridium bifermentans ATCC 19299): good to very good
Growth (Clostridium perfringens ATCC 13124 (WDCM 00007)): good to very good
Growth (Clostridium perfringens ATCC 10543): good to very good
Growth (Clostridium sporogenes ATCC 11437): good to very good
Growth (Clostridium sporogenes ATCC 19404 (WDCM 00008)): good to very good
Blacking (Escherichia coli ATCC 25922 (WDCM 00013)): -
Blacking (Bacillus cereus ATCC 11778 (WDCM 00001)): -
Blacking (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): -
Blacking (Clostridium bifermentans ATCC 19299): +
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007)): +
Blacking (Clostridium perfringens ATCC 10543): +
Blacking (Clostridium sporogenes ATCC 11437): +
Blacking (Clostridium sporogenes ATCC 19404 (WDCM 00008)): +
Incubation: 7 days; 30 °C; layered with paraffin.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
