Description
General description
Fluorocult® Lauryl sulfate broth + MUG (acc. ISO 11866 and FDA-BAM) is used to examine Escherichia coli in food, milk, and milk products. The lauryl sulfate inhibits the growth of undesirable microbial flora. The presence of E. coli is indicated by fluorescence under a long-wavelength UV lamp. A positive indole reaction and gas formation due to the fermentation of lactose confirm the results.
Application
Fluorocult® Lauryl sulfate broth + MUG is suitable for the examination of E. coli in food, milk, and milk products.
Features and Benefits
Fluorocult® fluorogenic culture media offer Rapid and Reliable culture testing
Powerful alternatives to conventional microbiological methods for the rapid and sensitive detection of bacteria
Offers superior granulated culture media
Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
Excellent wettability, solubility, and free-flowing properties
Convenient, with minimal component separation and clumping, even under warm or humid conditions
High batch-to-batch reproducibility
Prolonged shelf life of up to five years
High number of test strains exceeding all regulatory demands
Analysis Note
Appearance (clearness): clear
Appearance (color): yellowish to yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium(Escherichia coli NCTC 13216 (WDCM00202)): ≤ 100
Inoculum on reference medium (Citrobacter freundii ATCC 43864 (WDCM 00006)): ≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1E+04
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1E+04
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth Escherichia coliATCC 25922 (WDCM 00013): good to very good
Growth (Escherichia coli NCTC 13216 (WDCM00202)): good to very good
Growth (Citrobacter freundii ATCC 43864 (WDCM 00006)): good to very good
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total to partial inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total to partial inhibition
Gas (Escherichia coli ATCC 8739 (WDCM 00012)): +
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Escherichia coli NCTC 13216 (WDCM 00202)): +
Gas (Citrobacter freundii ATCC 43864 (WDCM 00006)): +
Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Gas (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Fluorescence (Escherichia coli ATCC 8739 (WDCM 00012)): +
Fluorescence (Escherichia coli ATCC 25922 (WDCM 00013)): +
Fluorescence (Escherichia coli NCTC 13216 (WDCM 00202)): +
Fluorescence (Citrobacter freundii ATCC 43864 (WDCM 00006)): -
Fluorescence (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Fluorescence (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Indole formation (Escherichia coli ATCC 8739 (WDCM 00012)): +
Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Indole formation (Escherichia coli NCTC 13216 (WDCM 00202)): +
Indole formation (Citrobacter freundii ATCC 43864 (WDCM 00006)): -
Indole formation (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Indole formation (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Incubation: 24 ± 2 to 48 ± 2 hours at 30 ± 1 °C, aerobic
Legal Information
FLUOROCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
