Description
General description
(mIFN- γ , NIH, reference standard, Gg 02–901-533, at least the same specific activity (EC 50 ) compared to the indicated standard is guaranteed.
Recombinant, Interferon-γ, mouse (mlFN-γ) is produced in E. coli and purified by standard chromatographic techniques.
Primary structure: One polypeptide chain (131 amino acids) identical to mouse, natural IFN- ? (133 amino acids), but lacking the last two amino acids at the carboxy-terminus and not glycosylated. Glycosylation is not essential for biological activity.
IFN-γ (interferon-γ) is produced by natural killer T cells, macrophages, Th1 CD4 T cells and activated CD8 T cells and the production increases with age.
Application
Recombinant mouse interferon-γ is a valuable tool for the study of IFN-γ (interferon-γ) actions in mouse model systems.
Interferon-γ, mouse (mIFN-γ) has been used in cell culture.
Biochem/physiol Actions
Species specificity: mouse
Interferon-γ (IFNγ) participates in cell-mediated immunity and has pro-inflammatory effects on immunocytes and target tissue. Its functions vary and is tissue specific. IFNγ induces T-cell-mediated immune response by increasing MHC-I expression on target cells and acts through IFNγ receptor, which is expressed on both immune and non-immune cells. IFNγ is associated with the development of colitis and Sjögren Syndrome.
Physical form
Solution, filtered through 0.2 μm pore size membrane, 100 000 U/ml (20 μg/ml) in PBS (phosphate buffered saline) and BSA (bovine serum albumin),1 mg/ml [purity of BSA: 98%, endotoxin (LAL): Purity: >95% (SDS-PAGE)
Endotoxin level:
Other Notes
EC 50 definition : The amount of mIFN- γ that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN-γ reference standard (Gg 02–901-533) (L cells-EMC virus/cytopathic effect) (1 unit equals =0.2 ng/ml).
Chain Length 131 AA
EC₅₀ definition: One unit is defined as the amount of mlFN-γ that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN-γ reference standard (Gg 02-901-533). (L cells-EMC virus/cytopathic effect) (1 unit equals ≤0.2 ng/ml).
For life science research only. Not for use in diagnostic procedures.
For life science research only. Not for use in diagnostic procedures.
1 EU corresponds to 0.1 ng.
The primary structure of recombinant Interferon-γ, mouse (mIFN-γ) is identical to that of natural, mouse IFN-γ (one polypeptide chain, 131 amino acids), however, recombinant mIFN-γ lacks the last two amino acids at the C-terminus and is not glycosylated. Glycosylation is not essential for biological activity.
