Merck, 113200-500UL, Anti-α-Actin Mouse mAb (1A4)

General description
Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Antibody Target Gene Symbol: ACTA2
Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
Hu Entrez ID: 59 (Related Antibodies: 113200 )
Rat Entrez ID: 81633

This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.
Immunogen
Human Smooth Muscle
a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
Application
Frozen Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
Analysis Note
Negative Control
Fibroblasts, striated muscle, or myocardium
Positive Control
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Other Notes
Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)