Merck, 11333062910, Anti-Digoxigenin

General description
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.
Contents: Lyophilizate
Application
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
ELISA
Immunohistocytochemistry
In situ hybridization
Western blot
Immunofluorescence staining
FISH (fluorescent in situ hybridization)

Biochem/physiol Actions
In the presence of Na⁺, Mg²⁺ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.
The monoclonal antibody reacts with free and bound digoxigenin.
Features and Benefits
Specific to free and bound digoxigenin
The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.

Preparation Note
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline: ELISA: for coating: 2 to 4 μg/ml
Immunohistocytochemistry: 0.5 to 2 μg/ml
In situ hybridization: 0.2 to 0.4 μg/ml
Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications
phosphate buffered saline, pH 7.4
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.
Analysis Note
There are no cross reactivities known.
Other Notes
For life science research only. Not for use in diagnostic procedures.