Merck, 1137270001, HPTLC plates, Silica gel 60 F₂₅₄ with concentrating zone 10 x 2.5 cm

General description
25 Glass plates 10 x 10 cm
These plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples. Our concentrating zone plates are based on different adsorption properties of two adsorbents. The first is a large pore concentrating adsorbent where the samples are applied; the second is a selective layer for separation. Regardless of the spots’ shape, size, or position, the sample always concentrates as a narrow band where the two adsorbents overlap and where the separation starts. These plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples.
Analysis Note
Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
layer thickness concentrating zone: 70 - 110 µm
Fluctuation in the thickness of the concentration zone: ≤ 35 µm
Chromatographic testing:
colour test
hRf-values
- bleu vif organol, colour test, lipophile:
18 - 24
- Ceres Black G, colour test, lipophile: 39 - 46
- ceres Violet brn, colour test, lipophile: 59 - 66
Typical value determined on a conditioned plate
Eluent: toluene (45% rel. Feuchte)
Other Notes
Replaces: 13727-5; 13727