Merck, 1137930001, PLC plates, Silica gel 60 F₂₅₄, 2 mm with concentrating zone 20 x 4 cm

General description
12 Glass plates 20 x 20 cm
These plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples. Our concentrating zone plates are based on different adsorption properties of two adsorbents. The first is a large pore concentrating adsorbent where the samples are applied; the second is a selective layer for separation. Regardless of the spots’ shape, size, or position, the sample always concentrates as a narrow band where the two adsorbents overlap and where the separation starts. These plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples.
Application

High-Performance Thin-Layer Chromatography (HPTLC): Silica gel 60 F₂₅₄ PLC plates are extensively used in HPTLC for separating and analyzing complex mixtures. These plates provide high resolution and selectivity for various analytes, including amino acids, peptides, and other organic compounds. Recent research highlights the effectiveness of these plates in separating dansyl derivatives of amino acids, demonstrating their importance in biochemical and clinical studies (Chomicki and Dzido, Current Issues in Pharmacy and Medical Sciences, 2022).

Analysis Note
Layer thickness: 1700 - 2100 µm
Deviation of layer thickness per plate: ≤ 300 µm
layer thickness concentrating zone: 700 - 1100 µm
Fluctuation in the thickness of the concentration zone: ≤ 200 µm
Chromatographic testing:
colour test
hRf-values
- bleu vif organol, colour test, lipophile:
20 - 30
- Ceres Black G, colour test, lipophile: 45 - 55
- ceres Violet brn, colour test, lipophile: 60 - 75
Typical value determined on a conditioned plate
Eluent: toluene (45% rel. humidity)
Other Notes
Replaces: 13793-7; 13793