Merck, 11388908910, Biotin-16-UTP

General description
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
Application
Biotin-labeled RNA can be used as a hybridization probe for:
Northern blots
Southern blots
Plaque or colony lifts
RNase protection experiments
In situ hybridization
Microarray hybridization
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
Physical form
Biotin-16-UTP, tetralithium salt, 10mM solution
Analysis Note
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
Other Notes
For life science research only. Not for use in diagnostic procedures.