Merck, 11426338910, Anti-Fluorescein-AP, Fab fragments

General description
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)
Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins.
Application
Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:
Dot blot
ELISA
Immunohistocytochemistry
In situ hybridization
Southern blot
Western blot
Electrochemical biosensing technique for the detection of 16S rRNA

Biochem/physiol Actions
The polyclonal antibody reacts with free and bound fluorescein.
Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).
Features and Benefits
Contents
Solution, stabilized
Preparation Note
Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
ELISA: 150 to 300mU/ml
Immunohistocytochemistry: 250 to 500mU/ml
In situ hybridization: 1.5 to 7.5U/ml
Southern blot: 150mU/ml
Western blot: 250 to 500 mU/ml

Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Other Notes
For life science research only. Not for use in diagnostic procedures.