| UNSPSC Code | 41115711 |
|---|---|
| ligand | (Sulfoethyl) |
| Quality Level | 400 |
| product line | Fractogel® |
| form | resin |
| specific analyte(s) | MAbs |
| parameter | 220 cm/hr flow rate |
| matrix active group | methacrylate |
| mean particle size | 40-90 μm |
| capacity | 160 mg binding capacity (lysozyme/ml of resin) |
| transition temp | flash point 35 °C (calculated) |
| application(s) | recombinant proteinvaccine development |
| separation technique | cation exchange |
| storage temp. | 2-30°C |
Answer:It is a strong cation exchanger with sulfoethyl functional groups.
Answer:This configuration contains 100 mL of resin.
Answer:The resin has a particle-size range of approximately 40–90 µm.
Fractogel® EMD SE Hicap (M) is a strong cation-exchange chromatography resin for purification of basic and neutral proteins, antibodies, peptides, and other biomolecules. This configuration contains 100 mL of resin suspended in 20% ethanol and 150 mM sodium chloride. The methacrylate matrix uses sulfoethyl functional groups and a 40–90 µm particle-size range. Tentacle technology provides a high accessible ligand density and a lysozyme binding capacity of up to approximately 160 mg/mL of resin. The medium is suited to recombinant-protein, monoclonal-antibody, and vaccine-development workflows that require high-capacity cation exchange with scalable process performance.
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