Merck, 11583816001, Anti-HA (12CA5)

General description
Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. The HA gene is mapped to segment 4 of influenza B viral genome.
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein.
Application
Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
Dot blots
Immunochemistry
Immunoprecipitation
Western blotting
Note: For experiments in which sensitivity is not critical, use Anti-HA (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity (3F10).
Biochem/physiol Actions
Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.
Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Preparation Note
11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Dot blot: 0.1 to 1 μg/ml
Immunfluorescence: 1-10 μg/ml
Western blot: 0.1 to 1 μg/ml

Analysis Note
Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.
Other Notes
For life science research only. Not for use in diagnostic procedures.