Merck, 1164640001, HPTLC plates, Silica gel 60 CN F₂₅₄s

General description
25 Glass plates 10 x 10 cm
Analysis Note
Specific surface area (BET): 460 - 520 m²/g
Pore volume: 0.71 - 0.78 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Steroidtest, straight phase
hRf-values
- hydrocortisone, straight phase:
10 - 20
- Reichstein S, straight phase: 20 - 30
- Methyltestosterone, straight phase: 35 - 47
B) Steroid test, reversed phase
- methyltestosterone, steroid test: 36 - 48
- Reichstein S, steroid test: 46 - 58
- hydrocortisone, steroid test: 56 - 68
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) petroleum benzine 40-60°/acetone (80/20 v/v)
Eluent b) acetone/water (30/70 v/v)

Other Notes
Replaces: 16464-5; 16464