Merck, 11718096001, DAB Substrate

General description
The metal enhanced DAB Substrate utilizes cobalt chloride and nickel chloride in a special formulation to produce a dark brown/black precipitate in the presence of horseradish peroxidase (HRP).
3, 3′ diaminobenzidine tetrahydrochloride (DAB) is a carcinogen that should be used safely. It is one of the major sensitive substrates for HRP (horseradish peroxidase). DAB is brown or amber in color and becomes insoluble and precipitates over the antigen-antibody site due to the presence of hydrogen peroxide and peroxidase.
Application
DAB Substrate is a color sustrate for peroxidases and is used in a variety of applications:Immunohistocytochemistry
In situ hybridization
Western blot
Dot blot

DAB substrate has been used in immunofluorescence and western blot.
Packaging
1 set containing 2 components.
Preparation Note
Working solution: Determine the volume of substrate required for the development of peroxidase.
Remove the DAB/metal concentrate (10x) from -15 to -25 °C storage.
Note: Do not bring to 15 to 25 °C.
Dilute the DAB/metal concentrate (10x) with the peroxide buffer to a 1x working solution. For example, if 5 ml of substrate are required, dilute 500 μl of the DAB/metal concentrate (10x) with 4.5 ml of the Peroxide buffer.
Mix well.
Add the 1x working solution to the tissue or blot until desired substrate development.
Note: The 1x solution is stable over many hours.
For best results, store the working solution at 2 to 8 °C when not using.

Storage conditions (working solution): 2 to 8 °C
For best results, store the working solution at 2 to 8 °C when not using.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Disclaimer
Supply conditions do not apply to the regions and states of Brazil: North, Northeast, Mato Grosso do Sul, Mato Grosso, and Rio Grande do Sul.