Merck, 11746081001, Nitrite/Nitrate, Colorimetric Test

General description
Linearity
The method is linear in the range from 0.02 mg nitrite or nitrate/l sample solution to 5.00 mg nitrite or nitrate/l sample solution.

Detection limit: 0.02mg/l for nitrite and nitrate
Application
Semimicro method for the determination of nitrite and/or nitrate to investigate water, waste water, environmental samples, plant material, foodstuffs, drugs, cosmetics, and biological samples. Nitrite/Nitrate, Colorimetric Test was used in a study done to estimate the in vivo effects of myrtle oil (Myrtii oleum) on the antioxidant enzymes such as catalase and superoxide dismutase in alloxan and normoglycaemic diabetic rabbits.
Biochem/physiol Actions
Under the provided conditions, nitrate reductase reacts specifically with nitrate ions.
Packaging
1 test combination containing 5 components.
Preparation Note
Working solution: Preparation of the solutions
Use the contents of bottle #1 undiluted, bring to 15 to 25 °C before use.
Place one Coenzyme-tablet in a beaker and dissolve in 3 ml of the solution from bottle 1. Remove the Coenzyme-tablet using the tweezers provided. The resulting solution is reaction mixture 2 and is sufficient for 12 nitrate determinations, bring to 15 to 25 °C before use.
Dissolve the contents of one bottle #3 in 0.7 ml of double-dist. water to give solution 3.
Use the contents of one bottle #4 undiluted.
Use the contents of one bottle #5 undiluted.

Storage conditions (working solution): Prepare Reaction Mixture 2 immediately before use.
Solution of Nitrate reductase is stable at 2 to 8 °C for 2 weeks.
Solution of Color reagent I is stable at 2 to 8 °C for 3 months.
Solution of Color reagent II is stable at 2 to 8 °C for 3 months.

Other Notes
For life science research only. Not for use in diagnostic procedures.