Merck, 11922416001, Anti-His<SUB>6</SUB>

General description
Anti-His6 is a monoclonal antibody to His6-tagged proteins. Anti-His6 specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the location of the histidine-tag in the protein sequence.
Application
Anti-His6 is used for the detection of His6-tagged proteins in:
Immunoblotting (e.g., dot blots and western blots)
Immunoprecipitation
Immunoassays (ELISA)
Immunocytochemistry
Immunofluorescence

Biochem/physiol Actions
Anti-His6 specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the histidine-tag in the protein sequence. In addition, Anti-His6 preferentially recognizes the C-terminal His6-epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.
Preparation Note
Stabilizers: BSA
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
ELISA: for detection 0.1 μg/ml; for coating 1 to 5 μg/ml
Immunoprecipitation: 0.5 to 5 μg/ml
Western blot: 0.2 to 0.5 μg/ml

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Other Notes
For life science research only. Not for use in diagnostic procedures.