General description293T cell line is a stable clone derivative of the human embryonic kidney (HEK) 293 cell line. It expresses the large T antigen of simian virus 40 (SV40).
Application293T cell line human has been used:
for lentiviral particle production
in the expression of eukaryotic proteins
for small interfering RNA (siRNA) transfection
for human immunodeficiency virus (HIV) transfection for pseudoviruses production
as a host for the transfection of pLKO.1.-TRC-ACE2 plasmid to study the localization and role of NL63-N, a nucleocapsid protein of human coronavirus NL63, in cell cycle progression
Biochem/physiol ActionsSTR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19
The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.
Due to the presence of T antigen, 293T cell line favors simian virus 40 (SV40) plasmid replication as well as high protein expression. It also possesses stem cell properties. HEK293T is used for large-scale vector production, displays increased cell growth and is efficient for transfection experiments when compared to HEK293 cells.
PackagingNOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA.
Preparation NoteDMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 10⁴ cells/cm².
Other NotesThis cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to
[email protected].
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