Merck, 121-25-02, Extracellular Matrix Protein Coated T-25 Flasks

Extracellular matrix protein-coated T-25 culture flasks provide a prepared attachment surface for adherent cells that benefit from extracellular matrix (ECM) contact. The 25 cm² culture area is pre-coated with Extracellular Matrix Attachment Factor, reducing the need to coat each flask manually before seeding. This configuration is useful for experimental culture of attachment-sensitive populations, including selected endothelial cells, when the coating is compatible with the cell-specific protocol. The attachment layer influences cell spreading and adherence, so experimental comparisons should use consistent surface preparation and avoid switching between coated and untreated vessels without evaluation. Each flask is a prepared culture vessel rather than a bottle of free ECM reagent. The product is marketed by Sigma-Aldrich and manufactured by Cell Applications, Inc. It is supplied for laboratory research, with cold handling and refrigerated storage relevant to preserving the prepared surface. Appropriate basal medium and supplements remain necessary for cell growth.