Merck, 1326570001, Alcian blue staining kit, pH 2.5

General description
Alcian blue pH 2.5 - for the detection of acidic mucosubstances - for microscopy, is a ready-to-use staining kit used to stain acidic mucopolysaccharides in human-histological specimen materials, for example histological sections of e. g. the intestine or stomach. Acidic mucosubstances are selectively stained in the tissue, after which the specimen is counterstained with nuclear fast red. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1. Counterstaining takes place with a nuclear fast red/aluminium sulfate solution. The package is sufficient for approx. 2500 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Application
For the detection of acidic mucosubstances.
For Microscopy.

Analysis Note
Nuclei: red
Acidic mucosubstances: light blue