Merck, 137715, 2,4,6-Tribromophenol
99%
Catalog Number: 137715
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Send an RFQ for Merck 137715, 2,4,6-Tribromophenol. Iright supports global sourcing and international shipment for life science and laboratory products.
| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | G8 mouse T cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA |
| Preparation | The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | PG - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions. To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions. Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. |
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| Additional Product Notes | TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq. The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation. View more applications data for this product in our Scientific Poster Library. |
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| Application References(PubMed link indicates BioLegend citation) | Dent AL, et al. 1990. Nature 343:714. Kelly KA, et al. 1993. Int. Immunol. 5:331. Sperling AI, et al. 1992. J. Immunol. 149:3200. Sperling AI, et al. 1997. J. Immunol. 159:86. |
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| RRID | AB_2860687 (BioLegend Cat. No. 137715) |
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| Structure | A member of Ig superfamily, associated with TCR δ chain and CD3 complex. |
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| Distribution | T cell subset in fetal and adult peripheral lymphoid tissues and thymus. |
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| Function | Might play a role in a previously unrecognized form of immunological surveillance for stress-induced self-antigens. |
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| Ligand/Receptor | Antigens |
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| Cell Type | T cells |
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| Biology Area | Adaptive Immunity, Immunology |
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| Molecular Family | TCRs |
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| Antigen References | 1. Allison JP, et al. 1991. Annu. Rev. Immunol. 9:679. 2. O’Brien RL, et al. 2000. J. Immunol. 165:6472. |
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| Gene ID | 110067 |
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| UniProt | View information about TCR Vgamma2 on UniProt.org |
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| Clone | UC3-10A6 |
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| Regulatory Status | RUO |
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| Other Names | Vγ2 T-cell receptor, T cell receptor gamma 2, TCR Vγ4 (Tonegawa nomenclature) |
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| Isotype | Armenian Hamster IgG |
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| Barcode Sequence | AAGCTGCACCGTAAT |
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