Merck, 14-731-M, CaM Kinase Iδ Protein, active, 20 µg

General description
N-terminal 6His-tagged, recombinant, human, full length CaM Kinase Iδ.
Recombinant human protein expressed by baculovirus in Sf21 cells.
Application
Active, N-terminal 6His-tagged, recombinant, human, full length CaM Kinase Iδ. For use in Enzyme Assays.
Research Category
Metabolism|Inflammation & Immunology
Research Sub Category
Obesity|Metabolic Disorders|Osteoporosis|Arthritis
Biochem/physiol Actions
Protein Target: CaMKIΔ
Target Sub-Family:CAMK

Physical form
Format: Ni2+/NTA-agarose
Presented as a frozen liquid in 50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Preparation Note
Stable for 6 months at -70°C from date of shipment. Upon first use, rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrog
Analysis Note
Routinely evaluated by phosphorylation of 150μM KKLRRTLSVA.
Other Notes
Concentration:For concentration, please refer to the Certificate of Analysis for individual lots of this enzyme.
For Specific Activity data, please refer to the Certificate of Analysis for individual lots of this enzyme.
For purity information, please refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial