Merck, 14-752-M, GRK7 Protein, active, 10 µg

General description
N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.
Recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.
Application
Active, N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. For use in Kinase Assays.
Research Category
Metabolism
Research Sub Category
Cardiovascular Disease|Metabolic Disorders
Biochem/physiol Actions
Protein Target: GRK7
Target Sub-Family:AGC

Physical form
10μg of recombinant protein in 100 μl of 50 mM Tris-HCl pH 8.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Format: Purified using Ni2+/NTA agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.
Preparation Note
Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Analysis Note
Routinely evaluated by phosphorylation of 2 mg/mL casein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of GRK7.
Other Notes
Specific Activity: where one unit of GRK7 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100μM.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial