Merck, 14-763-M, Tie2 (R849W) Protein, active, 250 µg

General description
42kDa
N-terminal 6His-tagged recombinant human Tie2 residues 771-end containing the R849W substitution.
The R849W mutation has been reported in dominantly inherited venous malformations. Expression studies in insect and mammalian cells demonstrates that Tie2 R849W has constitutively elevated tyrosine kinase activity compared with wild type. (Morris P.N., et al., Biochem.Biophys.Res.Commun.(2006);346:335-338 and Vikkula M, et al., Cell(1996);87:1181-1190).
Packaging
Also available in 10 μg size (cat#14-763) and in bulk (cat# 14-763-K).
Physical form
50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Preparation Note
Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap. Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Analysis Note
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.