Merck, 14-926-M, IKKε Protein, active, 10 µg

General description
IKBKE is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
Product Source: N-terminal FLAG and GST-tagged, recombinant, human IKK full length, expressed by baculovirus in Sf21 insect cells.
Application
Research Category
Signaling
Biochem/physiol Actions
Protein Target: IKKe
Target Sub-Family: Misc
Packaging
Also available in 250μg size as catalog # 14-926M and in bulk as catalog # 14-926-K.
Physical form
50mM Tris/HCl pH7.5, 150mM NaCl, 10mM β-glycerophosphate, 1mM sodium orthovanadate, 1mM NaF, 28% v/v glycerol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol
Purified by immunoaffinity chromatography.
Preparation Note
6 months at -70°C
Analysis Note
Routinely evaluated by phosphorylation of casein.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.