Merck, 14-935-M, GRK1, active

General description
Product Source: N-terminal 6His-tagged, recombinant, human GRK1 full length, expressed by baculovirus in Sf21 insect cells
Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
Biochem/physiol Actions
Target Sub-Family: AGC
Packaging
Also available in 250μg size as catalog # 14-935M and in bulk as catalog # 14-935-K.
Physical form
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Purified using immobilized metal affinity chromatography.
Preparation Note
6 months at -70°C
Analysis Note
Routinely evaluated by phosphorylation of KKKKERLLDDRHDSGLDSMKDEE
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.