Merck, 17112-500G, A1 Broth

General description
A1 Broth is a powdered microbiological medium designed for the selective detection of fecal coliforms, including Escherichia coli. A1 Broth is formulated with casein enzymic hydrolysate, providing nitrogen, amino acids, and peptides essential for bacterial growth, while lactose and salicin serve as fermentable carbohydrates to support acid and gas production for coliform identification. Sodium chloride maintains osmotic balance, ensuring cell stability, and Triton X-100 acts as a surfactant to inhibit non-target organisms and enhance nutrient dispersion. Together, these components create a selective and nutrient-rich environment ideal for detecting fecal coliforms.
Application
A1 Broth is widely used for detecting fecal coliforms, including Escherichia coli, in water quality monitoring and food safety testing using the Most Probable Number (MPN) method. It is applied in environmental testing of wastewater, seawater, and other samples for coliform contamination, as well as in food and beverage testing to ensure microbiological safety.
Features and Benefits
NutriSelect® Plus is a powdered culture media offering

Quality control with growth promotion test according to industry standards
Quality assurance acc. to ISO 9001
Cost-efficient powdered media

Preparation Note
Suspend 31.5 g in 1 litre distilled water. Heat to dissolve the medium completely. Distribute 10 ml amounts into tubes containing inverted Durham′s tubes. Sterilize by autoclaving at 121°C for 10 minutes.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany