Merck, 172104-50MG, Anaphase-Promoting Complex Inhibitor, TAME

General description
TAME is a potent ubiquitin ligase inhibitor that specifically blocks Anaphase-Promoting Complex (APC) activation by directly binding to APC and disrupts the IR-tail-dependent interactions between APC and its activator proteins Cdc20 or Cdh1. It stabilizes APC substrates and is shown to inhibit cyclin proteolysis in mitotic Xenopus egg extract with an IC₅₀ = 12 µM.
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Sackton, K.L., et al. 2014. Nature. In press.
Zeng, X., et al. 2010. Cancer Cell18, 382.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)