Merck, 196418-1GM, BAPTA, Tetrasodium Salt

General description
Ca²⁺-chelator exhibiting a 10⁵-fold greater affinity for Ca²⁺ (110 nM) than for Mg²⁺. After BAPTA complexes with Ca²⁺, the UV spectrum shifts from 254 to 279 nm at pH >6.0. Useful for spectrophotometric monitoring of extracellular Ca²⁺ levels, especially transient phenomena, because of its insensitivity to pH. Has been used for the measurement and control of Ca²⁺ levels in eggs, chromaffin cells, muscle cells, retinal cells, and pancreatic cells. In conjunction with certain formazan dyes, BAPTA can be used in a colorimetric assay for Mg²⁺. Has neuroprotective effects in vitro against excitotoxic damage and spontaneous recurrent seizures, such as epilepsy.
Preparation Note
Following reconstitution, aliquot, purge with inert gas and freeze (-20°C). To increase stability in solution, store at a high pH (>10). Stock solutions are stable for up to 1 month at -20°C.
Other Notes
DeLorenzo, R.J., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14482.
Li, L., et al. 1995. J. Cell Physiol. 163, 105.
Tymianski, M., et al. 1994. J. Neurophysiol. 72, 1973.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)