Merck, 217356-1MG, Carboxypeptidase B, Porcine Pancreas

General description
Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn²⁺ chelators. Has an optimal pH of 7.0-9.0 and a pI of 6.0.
Physical form
Lyophilized from 360 µM HEPES, 1.41 µM zinc chloride, 1.41 mg/ml Trehalose, pH 7.4.
Preparation Note
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Folk, J.E. 1970. Methods Enzymol.19, 504.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Hip-Arg per min at 25°C, pH 7.7.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)