Merck, 217594-100UL, Anti-CD44 Rat mAb (A020)

General description
Anti-CD44, rat monoclonal, clone A020, recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 in mouse. It is validated for ELISA, IF, IP, RIA, WB, and IHC.
Rat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.
Recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.
Immunogen
Human
purified human lymphocyte CD44
Application
ELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:1000)
Immunofluorescence (1:200)
Immunohistochemistry (1:250)
Immunoprecipitation (1:200)
Neutralization Studies (see comments and application references)
Radioimmunoassay (1:5000)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol.162, 121.
Bourguignon, L.Y.W., et al. 1993. J. Immunol.151, 6634.
The antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)