Merck, 218785-3000U, Caspase-10, Human, Recombinant, E. coli

General description
M.W. 11,000 and 18,000 (heterodimer).
Recombinant, human caspase-10 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
Physical form
In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Kang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.
One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)