Merck, 218799, Caspase-6, Human, Recombinant, E. coli

General description
M.W. 11,000 and 18,000 (heterodimer).
Recombinant, human caspase-6 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Reconstitute to 1 unit/µl in PBS, 15% glycerol.
Other Notes
LeBlanc, A., et al. 1999. J. Biol. Chem.274, 23426.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.
One unit is defined as the amount of enzyme that will cleave 1 nmol Ac-VEID-pNA (Cat. No. 218762) per h at 37°C, pH 7.2.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)