Merck, 218800, Caspase-7, Human, Recombinant, E. coli

General description
M.W. 12,000 and 20,000 (heterodimer).
Recombinant, human caspase-7 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 12000 and 20000.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C). Stock solitions are stable for up to 3 months at -70°C.
Reconstitute to 1 unit/µl in PBS, 15% glycerol.
Other Notes
One unit is defined as the amount of enzyme that will cleave 1 nmol of Ac-DEVD-pNA (Cat. No. 235400) per hour at 37°C, pH 7.2.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)