Merck, 21888-25MG-F, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

General description
5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.
Application
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. Forms green fluorescent conjugates on deacetylation.
Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.
5-Carboxyfluorescein diacetate N-succinimidyl ester has been used:
to label cells for flow cytometry
in lymphocyte proliferation assay
to determine the intracellular or intra-organellar pH in chloroplasts
to label Leishmania amazonensis and Leishmania major

Biochem/physiol Actions
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.