Merck, 219415-1MG, Cathepsin Inhibitor I

General description
Cell-permeable cysteine protease inhibitor. Inhibits cathepsin B (k₂/Ki = 8.9 x 10³ M⁻¹ sec⁻¹), cathepsin L (k₂/Ki = 3.8 x 10⁵ M⁻¹ sec⁻¹), cathepsin S (k₂/Ki = 4.2 x 10⁴ M⁻¹ sec⁻¹), and papain (k₂/Ki = 2.4 x 10³ M⁻¹ sec⁻¹).
Cysteine protease inhibitor. Selectively inhibits cathepsin B (k₂/Ki = 8.9 x 10³ M⁻¹sec⁻¹), cathepsin L (k₂/Ki = 3.8 x 10⁵ M⁻¹sec⁻¹), cathepsin S (k₂/Ki = 4.2 x 10⁴ M⁻¹sec⁻¹), and papain (k₂/Ki = 2.4 x 10³ M⁻¹sec⁻¹). Half-life in buffered solution (pH 5-9) = 25 h (30°C); 300 h (0°C).
Half-life in buffered solution (pH 5-9) = 25 h (30°C); 300 h (0°C).
Biochem/physiol Actions
Cell permeable: yes
Primary Target
cathepsin B
Product does not compete with ATP.
Reversible: no
k₂/Ki = 8.9 x 10³ M⁻¹sec⁻¹ for cathepsin B
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Z-Phe-Gly-NHO-Bz
Zhu, D. and Uckun, F.M. 2000. Clin. Cancer Res.6, 2064.
Demuth, H.-U., et al. 1996. Biochim. Biophys. Acta 1295, 179.
Brömme, D., and Demuth, H.-U. 1994. Methods Enzymol. 244, 671.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)