Description
General description
Native coagulation factor Xa from human plasma prepared from homogeneous Factor X by activation with Russel’s viper venom. The venom is removed after activation. During coagulation, in the presence of Ca²⁺ and membrane phospholipids, a complex of Factor Xa and Factor Va (the prothrombinase complex) activates prothrombin to thrombin. Inhibitors include AEBSF (Cat. No. 101500 ), DFP (Cat. No. 30967), PMSF (Cat. No. 52332 ), and Soybean Trypsin Inhibitor (Cat. No. 650357 ). Sold on the basis of plasma equivalent units of Factor X. 100 plasma equivalent units are equal to 800 µg of Factor X.
Physical form
Lyophilized from 700 mM NaCl, 20 mM Tris-HCl, pH 7.4.
Analysis Note
homogeneous by SDS-PAGE
Other Notes
Heeb, M.J., et al. 1994. Proc. Natl. Acad. Sci. USA91, 2728.
Lindhout, T., et al. 1994. Biochem. J.297, 131.
Morishita, H., et al. 1994. Thrombosis Res.73, 193.
Lupu, C., et al. 1993. Thrombosis Haemostasis70, 579.
Jordan, S.P., et al. 1992. Biochemistry31, 5374.
Schoen, P., et al. 1992. Br. J. Haemotol.81, 255.
Tracy, P.B., et al. 1992. Methods Enzymol.215, 329.
Lawson, J.H., and Mann, K.G. 1991. J. Biol. Chem.226, 11317.
Tans, G., et al. 1991. J. Biol. Chem.266, 21864.
Owen, W.G., et al. 1974. J. Biol. Chem.249, 594.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol CHROMOZYM® X substrate per min at 25°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Chromozym is a registered trademark of Pentapharm AG, Basel
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Toxicity: Standard Handling (A)
